Tramadol hydrochloride and Diclofenac sodium are used in combined dosage form for used for the symptomatic treatment of moderate to severe pain in adult patients. Present work consist development and validation of HPTLC estimation of Tramadol hydrochloride (TRA) and Diclofenac sodium (DCS) in Synthetic mixture. These UV spectroscopy methods are developed using CAMAG loaded with Win Cats software.for wavelengths were selected 280 nm for TRA and DCS as Toluene,Ethylacetate,Methanol and Ammonia used as a solvent. In this HPTLC methods linearity of TRA and DCS were found to be in range of 2500-7500 ng/spot and 3750-11250 ng/spot respectively with correlation coefficient > 0.998. this method are simple, accurate, precise, economical and statistically validated. There was no interference of any excipient in the determination of drugs in synthetic mixture. These method can be applied for routine quality control analysis and dissolution study.
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